Tridot Test - Preparation, Uses & Procedure

The Tridot Test is a HIV Rapid Card diagnostic method used to detect the presence of certain antibodies in the blood, often employed in screening for infections and diseases. This blog provides a comprehensive overview of the Tridot Test, including its purpose, preparation steps, and detailed procedure and medical equipment used. It explores the various applications of the Tridot Test, outlines the associated costs, and highlights different brands available on Medikabazaar.

What is a HIV Tridot Test?

Human Immunodeficiency Virus (HIV) comprises two types: HIV-1, the most widespread globally, and HIV-2, which is less common and primarily found in Western Africa. The HIV-1 type typically tests positive more quickly, while HIV-2 may not show positive results as readily. The TRIDOT HIV test is a rapid, visual, and precise immunoassay designed to differentiate between HIV-1 and HIV-2 antibodies (IgM, IgG, and IgA) in human serum or plasma. This screening test uses HIV-1 and HIV-2 antigens immobilized on an immunofiltration membrane to detect antibodies. The test works by having the patient's sample pass through the membrane, where HIV antibodies bind to the antigens and produce distinct pinkish-purple dots, providing a visual result against a white background.

Preparation for Tridot Test:

HIV antigens are immobilized on a porous immunofiltration membrane within the test device. As the sample and reagents pass through the membrane, HIV antibodies, if present, bind to these immobilized antigens. A conjugate then binds to the Fc portion of the HIV antibodies, producing distinct pinkish-purple dots against a white background.

Test Requirements:

Following are the steps for preparation for the tridot test.

Test Kit Contains:

• HIV TRI-DOT test device
• Buffer solution
• Protein-A conjugate
• Sample dropper

Additional Items Needed:

• Infectious waste bin
• Gloves
• Timer
• Specimen: serum/plasma

Tridot Test Procedure:

1. Add 3 drops of buffer solution to the center of the test device.
2. Using the sample dropper, add 1 drop (50µl) of the patient's serum or plasma to the device. Use a separate dropper for each specimen.
3. Add 5 drops of buffer solution to the device.
4. Add 2 drops of Protein-A conjugate from the vial.
5. Add 5 more drops of buffer solution and read the results immediately.
6. Discard the device in the infectious waste bin after reading the results.

Different Uses of Tridot Test:

Screening for HCV Infection: The test is used to screen individuals for hepatitis C by detecting antibodies produced in response to an HCV infection.

Diagnosis of Hepatitis C: A positive result indicates potential exposure to HCV. Further tests, such as polymerase chain reaction (PCR), are needed to confirm an active infection and determine viral load.

Monitoring Treatment Progress: For patients undergoing HCV treatment, the test helps assess the effectiveness of antiviral therapy by monitoring changes in antidiv levels over time.

Preventive Measures: Early detection through the HCV Tri-Dot test allows for timely counseling and preventive actions, helping to prevent the spread of the virus and manage liver disease progression.

Cost of a Tridot Test:

• Costs can vary by region. In metropolitan areas such as Mumbai, Delhi, Kolkata, or Pune, where hepatitis C prevalence is higher, prices might be slightly elevated.
• The test price often covers laboratory fees, sample collection charges, and professional fees. It’s crucial to understand what is included in the cost to avoid unexpected expenses.
• Higher-quality laboratories with advanced facilities and stringent quality control measures may charge more. Choosing a certified lab known for accuracy and reliability is advisable.
• Healthcare centers sometimes offer promotional offers or discounts on diagnostic tests, which can reduce overall costs. Checking for such offers can help you get a better understanding of the test range and save money.

Different Brands of Tridot Test on Medikabazaar:

Explore reputed brands like Abbot, Polymed, Pathkits and many more only at Medikabazaar.

Alpine 1/2 HIV Rapid Card: Provides a quick screening method for detecting HIV-1 and HIV-2 antibodies, allowing for fast results with minimal equipment.

Abbott HIV 1/2 3.0 CE (D) Global Rapid Card: A globally recognized test card offering reliable and rapid detection of HIV-1 and HIV-2 antibodies, with CE mark certification for quality assurance.

Polymed HIV 1/2 Antibody Detection Kit: A user-friendly rapid card designed for detecting HIV-1 and HIV-2 antibodies, suitable for various testing environments.

J. Mitra HIV Tri-Dot Rapid Card: Utilizes a visual, three-dot format to distinguish between HIV-1 and HIV-2 antibodies, providing clear and rapid results.

Pathkits Simple 1 & 2 HIV Rapid Card: A straightforward testing card for detecting HIV-1 and HIV-2 antibodies, designed for ease of use and quick results.

In summary, HIV rapid test cards are crucial tools in the swift and effective detection of HIV-1 and HIV-2 antibodies. Each test card, including those from Alpine, Abbott, Polymed, J. Mitra, and Pathkits, offers unique features and reliability for screening and diagnosis. These tests provide crucial information for early intervention and management of HIV, enhancing overall public health efforts.

Your Goto Medical Supplies App for Lab Products

Download Medikabazaar App for buying the product you need. You can choose from a variety of products on our app.